Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana
Biomolecules & Therapeutics
; : 334-340, 2014.
Article
de En
| WPRIM
| ID: wpr-199227
Bibliothèque responsable:
WPRO
ABSTRACT
In this study, 23 oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. The NF-kappaB inhibitory activity of the isolated compounds was measured in TNFalpha-treated HepG2 cells using a luciferase reporter system. Compounds 19-23 inhibited TNFalpha-stimulated NF-kappaB activation in a dose-dependent manner, with IC50 values ranging from 0.75-8.30 microM. Compounds 19 and 20 also inhibited the TNFalpha-induced expression of iNOS and ICAM-1 mRNA. Moreover, effect of the isolated compounds on PPARs transcriptional activity was assessed. Compounds 7-11 and 19-23 activated PPARs the transcriptional activity significantly in a dose-dependent manner, with EC50 values ranging from 0.9-10.8 microM. These results suggest the presence of potent anti-inflammatory components in P. koreana, and will facilitate the development of novel anti-inflammatory agents.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Saponines
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ARN messager
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Facteur de transcription NF-kappa B
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Facteur de nécrose tumorale alpha
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Molécule-1 d'adhérence intercellulaire
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Concentration inhibitrice 50
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Pulsatilla
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Récepteurs activés par les proliférateurs de peroxysomes
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Méthanol
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Cellules HepG2
langue:
En
Texte intégral:
Biomolecules & Therapeutics
Année:
2014
Type:
Article