Induction of Potent Antigen-specific Cytotoxic T Cell Response by PLGA-nanoparticles Containing Antigen and TLR Agonist
Immune Network
; : 30-33, 2013.
Article
de En
| WPRIM
| ID: wpr-199843
Bibliothèque responsable:
WPRO
ABSTRACT
Previously we showed that biodegradable nanoparticles containing poly-IC or CpG oligodeoxynucleotide (ODN) together with ovalbumin (OVA) were efficient at inducing MHC-restricted presentation of OVA peptides in dendritic cells. The CTL-inducing activities of the nanoparticles were examined in the present study. Nanoparticles containing poly-IC or CpG ODN together with OVA were prepared using biodegradable polymer poly(D,L-lactic acid-co-glycolic acid), and then were opsonized with mouse IgG. The nanoparticles were injected into the tail vein of mice, and 7 days later the OVA-specific CTL activities were measured using an in vivo CTL assay. Immunization of mice with the nanoparticles containing poly-IC or CpG ODN together with OVA elicited potent OVA-specific CTL activity compared to those containing OVA only. In accordance with these results, nanoparticles containing poly-IC or CpG ODN together with OVA exerted potent antitumor activity in mice that were subcutaneously implanted with EG7.OVA tumor cells. These results show that encapsulation of poly-IC or CpG ODN together with antigen in biodegradable nanoparticles is an effective approach for the induction of potent antigen-specific CTL responses in vivo.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Ovule
/
Peptides
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Acide polyglycolique
/
Polymères
/
Veines
/
Cellules dendritiques
/
Immunoglobuline G
/
Ovalbumine
/
Immunisation
/
Acide lactique
Limites du sujet:
Animals
langue:
En
Texte intégral:
Immune Network
Année:
2013
Type:
Article