Role of zinc for calcification inhibitor protein in vascular smooth muscle cell plaque formation / 한국영양학회지
Journal of Nutrition and Health
; : 59-62, 2016.
Article
Dans Ko
| WPRIM
| ID: wpr-201519
Responsable en Bibliothèque :
WPRO
ABSTRACT
PURPOSE:
Zinc, a biomineral present within and outside cells, manages various cellular mechanisms. In this study, we examined whether zinc was involved in vascular smooth muscle cell (VSMC) calcification via regulation of calcification inhibitor protein, osteopontin (OPN).METHODS:
Rat aorta cell line (A7r5 cells) and primary vascular smooth muscle cells (pVSMCs) from rat aorta were cultured with phosphate (1-5 mM) and zinc (0-15 microM) as appropriate, along with osteoblasts (MC3T3-E1) as control. The cells were then stained for Ca and P deposition for calcification examination as well as osteopontin expression as calcification inhibitor protein was measured.RESULTS:
Both Ca and phosphate deposition increased as the addition of phosphate increased. In the same manner, the expression of osteopontin was upregulated as the addition of phosphate increased in both cell types. When zinc was added, Ca and P deposition decreased in VSMCs, while it increased in osteoblasts.CONCLUSION:
The results imply that zinc may prevent VSMC calcification by stimulating calcification inhibitor protein OPN synthesis in VSMCs.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Aorte
/
Ostéoblastes
/
Zinc
/
Lignée cellulaire
/
Ostéopontine
/
Muscles lisses vasculaires
Limites du sujet:
Animals
langue:
Ko
Texte intégral:
Journal of Nutrition and Health
Année:
2016
Type:
Article