Evaluation of immunocontraceptive vaccine composed of gonadotropin-releasing hormone conjugated with granulocyte-macrophage colony-stimulating factor in male rats
Korean Journal of Veterinary Research
; : 155-158, 2017.
Article
Dans En
| WPRIM
| ID: wpr-211709
Responsable en Bibliothèque :
WPRO
ABSTRACT
Surgical castration performed to reduce male-associated problems results in pain and microbial infections in male animals. Therefore, immunocontraception, which is mediated by the animal's own antibodies against reproductive hormones, has been recommended as an alternative to surgical castration when considering the animal's welfare. In this study, a new immunocontraceptive vaccine composed of six tandem copies of gonadotropin-releasing hormone (GnRH) fused to rat granulocyte-macrophage colony-stimulating factor (GM-CSF) was developed, and its efficacy was evaluated in male rats. Three different doses (10, 50, and 100 µg) of recombinant GM-CSF–GnRH protein were injected three times at intervals of two weeks into male rats. The rats vaccinated with three doses of GM-CSF–GnRH produced a significantly higher level of antibodies against GnRH than that in the negative control rats. Severe atrophy of gonads was observed in rats vaccinated with three doses of GM-CSF–GnRH but not in the negative control rats. The results reveal that the new GnRH vaccine conjugated with rat GM-CSF induces efficient immunocontraception in male rats. This formulation of the immunocontraceptive vaccine would be applicable to both domestic and pet male animals.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Atrophie
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Vaccins
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Castration
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Facteur de stimulation des colonies de granulocytes et de macrophages
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Hormone de libération des gonadotrophines
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Immunocontraception
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Gonades
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Anticorps
Limites du sujet:
Animals
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Humans
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Male
langue:
En
Texte intégral:
Korean Journal of Veterinary Research
Année:
2017
Type:
Article