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Analysis of Cellular Changes Resulting from Forced Expression of Dickkopf-1 in Hepatocellular Carcinoma Cells / Journal of the Korean Cancer Association, 대한암학회지
Article de En | WPRIM | ID: wpr-212924
Bibliothèque responsable: WPRO
ABSTRACT
PURPOSE: Recent studies have shown that Dickkopf-1 (DKK-1) is overexpressed in some tumors, including hepatocellular carcinoma. However, the role of increased DKK-1 in these tumors is not known. In this study, the DKK-1 expression in hepatocellular carcinoma (HCC) cell lines was evaluated and the effect of DKK-1 overexpression in HCC cell lines was studied. MATERIALS AND METHODS: The expression of DKK-1 in hepatocellular carcinoma cell lines was evaluated by RT-PCR. Stable cell lines that overexpressed DKK-1 were established. Cell growth, adhesion, migration and invasion assays were performed. RESULTS: RT-PCR analysis showed that 5 out of 8 HCC cell lines expressed DKK-1. The forced expression of DKK-1 suppressed the growth of cells and increased the population of cells in the sub-G1 phase. In addition, DKK- 1 reduced the cellular adhesion capacity to collagen type I and fibronectin, and it increased migratory capacity. However, overexpression of DKK-1 did not increase the invasion capacity of the HCC cell line. CONCLUSION: Collectively, our data suggest that overexpression of DKK-1 affects the biology of HCC cells.
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Texte intégral: 1 Indice: WPRIM Sujet Principal: Biologie / Adhérence cellulaire / Lignée cellulaire / Fibronectines / Apoptose / Carcinome hépatocellulaire / Collagène de type I langue: En Texte intégral: Cancer Research and Treatment Année: 2007 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Biologie / Adhérence cellulaire / Lignée cellulaire / Fibronectines / Apoptose / Carcinome hépatocellulaire / Collagène de type I langue: En Texte intégral: Cancer Research and Treatment Année: 2007 Type: Article