The Effect of Terazosin on the Histological Changes of Rat Bladder after Partial Outlet Obstruction

ABSTRACT

PURPOSE: When the bladder outlet is partially obstructed, The expression of iNOS and collagen type III is caused by ischemia of bladder. This study aimed to evaluate the hemodynamic changes and the expression of inducible nitric oxide synthase(iNOS) and collagen type III of bladder during acute stages of partial bladder outlet obstruction in female rats and conducted a research on the effect of alpha-adrenergic blocker. MATERIALS AND METHODS: Thirty mature Sprague-Dawley rats were randomly divided into three groups; 3 days(Group I), 7 days(Group II), 14 days(Group III), depending on the term of partial bladder outlet obstruction. After obstruction, each group was subdivided into the experimental groups and the control groups; terazosin(0.4 mg/kg) was administered to the experimental groups and normal saline was administered to the control groups for a week. The degree of expression of iNOS and collagen type III in bladder was investigated by immunohistochemical stain and Western blot. RESULTS: The blood flow of experimental groups showed significant increase compared to control groups(p<0.05). The expression of iNOS and collagen type III of exerimental groups was significantly decreased compared to the controls(p<0.05). CONCLUSION: This study suggests that alpha-adrenergic blocker makes increase in blood flows to bladder and may have a role of prevention from functional damage of bladder.