Interaction of Wnt5a with Notch1 is Critical for the Pathogenesis of Psoriasis
Annals of Dermatology
; : 45-54, 2016.
Article
Dans En
| WPRIM
| ID: wpr-223552
Responsable en Bibliothèque :
WPRO
ABSTRACT
BACKGROUND:
Psoriasis is characterized by uncontrolled hyperproliferation, aberrant differentiation, and dermal infiltration of immune cells. Recent studies have reported that Wnt5a and Notch1 signaling are altered in psoriatic skin lesions.OBJECTIVE:
We aimed to investigate the interaction of Wnt5a with Notch 1 with respect to inflammation-mediated epidermal hyperproliferation in psoriasis.METHODS:
Expression of Wnt5a and Notch1 signaling-related proteins were examined in psoriatic skin biopsies. Wnt5a was upregulated in human keratinocytes by treating the cells with its recombinant form (rWnt5a).RESULTS:
In psoriatic lesions, expression of Wnt5a increased while that of Notch1 decreased when compared to that in non-lesional and normal skin. Treatment with rWnt5a increased the proliferation of keratinocytes and increased their secretion of interleukin (IL)-23, IL-12, and tumor necrosis factor (TNF)-alpha. Further, exposure of keratinocytes to IL-1alpha, TNF-alpha, transforming growth factor-alpha, and interferon-gamma downregulated Notch1 as well as HES 1, which is downstream to Notch1, but increased the Wnt5a levels. The upregulated Wnt5a in keratinocytes downregulated both Notch1 and HES1.CONCLUSION:
Our data suggest that Wnt5a and Notch1 signaling exert counteracting influences on each other and are involved, in part, in the pathomechanism of psoriasis.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Psoriasis
/
Peau
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Biopsie
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Kératinocytes
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Interleukines
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Interféron gamma
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Facteur de nécrose tumorale alpha
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Interleukine-12
Type d'étude:
Etiology_studies
Limites du sujet:
Humans
langue:
En
Texte intégral:
Annals of Dermatology
Année:
2016
Type:
Article