Association of Single-Nucleotide Polymorphisms of the MBL2 with Atopic Dermatitis in Korean Patients
Annals of Dermatology
; : 571-577, 2017.
Article
de En
| WPRIM
| ID: wpr-226485
Bibliothèque responsable:
WPRO
ABSTRACT
BACKGROUND: Human mannose-binding lectin (MBL) is a serum lectin taking part in the innate immunity by opsonizing various microorganisms for phagocytosis. The MBL serum concentration is affected by several single-nucleotide polymorphisms (SNPs) in the promoter region of the MBL2 gene. OBJECTIVE: The purpose of this study was to examine the relationship between MBL2 polymorphisms and atopic dermatitis (AD) susceptibility. METHODS: To examine whether the MBL2 SNPs are related to AD susceptibility, we examined 237 patients with AD and 94 controls by polymerase chain reaction (PCR)-restriction fragment length polymorphism and PCR-sequence specific primer analyses of four polymorphic loci: two (H/L and X/Y) within the promoter region and the other two (P/Q and A/B) within exon 1. MBL concentrations in the blood were estimated by ELISA. RESULTS: The prevalence of haplotype HYPB, leading to MBL deficiency, was significantly decreased in the AD patients compared to the controls (p=0.002), while the prevalence of haplotype HYPA was increased with a clear trend toward significance (p=0.056). The frequency of MBL2 LYPB/LXPA (odds ratio, 0.08; 95% confidence interval, 0.009~0.655; p=0.021) were significantly decreased in the AD patients. The blood log [total immunoglobulin E, IgE] levels of MBL2 HYPA/HYPA, HYPA/LYPA, HYPA/LYPB, HYPA/LYQA, and LYQA/LXPA haplotype pairs were significantly increased in the AD patients. CONCLUSION: The frequency of MBL2 HYPB haplotype was significantly decreased in the AD patients compared to the controls. The frequency of LYPB/LXPA had a possibly protective effect on AD. Moreover, the MBL2 HYPA haplotype pairs, which were related to higher blood total IgE levels, were possibly associated with extrinsic AD.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phagocytose
/
Haplotypes
/
Immunoglobuline E
/
Immunoglobulines
/
Test ELISA
/
Réaction de polymérisation en chaîne
/
Exons
/
Prévalence
/
Régions promotrices (génétique)
/
Polymorphisme de nucléotide simple
Type d'étude:
Prevalence_studies
Limites du sujet:
Humans
langue:
En
Texte intégral:
Annals of Dermatology
Année:
2017
Type:
Article