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The building of chip-based capillary electrophoresis platform and application / 中华医学遗传学杂志
Article de Zh | WPRIM | ID: wpr-229837
Bibliothèque responsable: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the point mutations by chip-based capillary electrophoresis and to provide a rapid and sensitive technique detection for beta-thalassemia.</p><p><b>METHODS</b>Multiplex primer-extension reaction was used to amplify the common loci of the samples for beta-thalassemia. The reaction products were detected by the chip-based capillary electrophoresis and the genotypes of the samples were discrened.</p><p><b>RESULTS</b>A system was constructed to detect the point mutations of beta-thalassemia by chip-based capillary electrophoresis, and the technology was ralidated by the patients' samples and the results coincided with those of detection kit.</p><p><b>CONCLUSION</b>Beta-thalassemia can be detected by chip-based capillary electrophoresis rapidly with a small amount of samples. It would be a new detection method of the genetic disorders.</p>
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Bêta-Thalassémie / Électrophorèse capillaire / Séquençage par oligonucléotides en batterie / Diagnostic / Génétique / Maladies génétiques congénitales / Méthodes Type d'étude: Diagnostic_studies Limites du sujet: Female / Humans / Male langue: Zh Texte intégral: Zhonghua Yi Xue Yi Chuan Xue Za Zhi Année: 2007 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Bêta-Thalassémie / Électrophorèse capillaire / Séquençage par oligonucléotides en batterie / Diagnostic / Génétique / Maladies génétiques congénitales / Méthodes Type d'étude: Diagnostic_studies Limites du sujet: Female / Humans / Male langue: Zh Texte intégral: Zhonghua Yi Xue Yi Chuan Xue Za Zhi Année: 2007 Type: Article