Effect of some active Chinese herbal fraction on brain tissue proteomic profile of ischemic mouse / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of some active Chinese herbal fraction on protein expression of brain tissue in ischemic mouse with proteomic technique.</p><p><b>METHODS</b>Ischemia-reperfusion mice were treated with baicalin, geniposide, cholic acid and concha margaritifera respectively for 3 hrs, and then their brain tissue were taken to extract the total protein. Protein expression in ischemic mouse brain was analyzed with surface-enhanced laser desorption/inionation-time of flight-mass spectra (SELDI-TOF-MS) protein-chip.</p><p><b>RESULTS</b>The four components tested had effect on 3 target proteins at 5373Da, 5707Da and 15103Da, showing the nature of multi-target and with different action on protein expression.</p><p><b>CONCLUSION</b>Protein-chip is an effective approach for exploring the pharmacological mechanism of Chinese herbal fraction.</p>