Effect of some active Chinese herbal fraction on brain tissue proteomic profile of ischemic mouse / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 526-528, 2006.
Article
Dans Zh
| WPRIM
| ID: wpr-230210
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of some active Chinese herbal fraction on protein expression of brain tissue in ischemic mouse with proteomic technique.</p><p><b>METHODS</b>Ischemia-reperfusion mice were treated with baicalin, geniposide, cholic acid and concha margaritifera respectively for 3 hrs, and then their brain tissue were taken to extract the total protein. Protein expression in ischemic mouse brain was analyzed with surface-enhanced laser desorption/inionation-time of flight-mass spectra (SELDI-TOF-MS) protein-chip.</p><p><b>RESULTS</b>The four components tested had effect on 3 target proteins at 5373Da, 5707Da and 15103Da, showing the nature of multi-target and with different action on protein expression.</p><p><b>CONCLUSION</b>Protein-chip is an effective approach for exploring the pharmacological mechanism of Chinese herbal fraction.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Encéphale
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Médicaments issus de plantes chinoises
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Lésion d'ischémie-reperfusion
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Chimie
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Spectrométrie de masse MALDI
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Infarctus encéphalique
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Analyse par réseau de protéines
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Protéomique
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Utilisations thérapeutiques
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Traitement médicamenteux
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Integrated Traditional and Western Medicine
Année:
2006
Type:
Article