Hairy-root culture of Polygonium multiflorum Thunb. and the production of its active constituents-anthraquinones / 生物工程学报

ABSTRACT

Transformed hairy roots of Polygonium multiflorum Thunb. were obtained by the transformation of Agrobacterium rhizogenes LBA9402. It was clearly demonstrated that T-DNA of A. Rhizogenes Ri plasmid was integrated into the cells of hairy roots by the experiments of PCR and Southern hybridization. Through the screen of basic medium and the research of growth curve of hairy roots, the optimum inoculum time was selected in 30 days or so in the optimum condition-MS medium. On the condition, the content of rhein was 2.85 folds higher in hairy roots than that of natural plants by means of HPLC.