Ectopic osteogenesis in vivo using bone morphogenetic protein-2 derived peptide loaded biodegradable hydrogel / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 811-815, 2014.
Article
Dans Zh
| WPRIM
| ID: wpr-234505
Responsable en Bibliothèque :
WPRO
ABSTRACT
We investigated the development of an injectable, biodegradable hydrogel composite of poly(trimethylene carbonate)-F127-poly(trimethylene carbonate)(PTMC11-F127-PTMC11 )loaded with bone morphogenetic protein-2 (BMP-2) derived peptide P24 for ectopic bone formation in vivo and evaluated its release kinetics in vitro. Then we evaluated P24 peptide release kinetics from different concentration of PTMC11-F127-PTMC11 hydrogel in vitro using bicinchoninic acid (BCA)assay. P24/ PTMC11-F127-PTMC11 hydrogel was implanted into each rat's erector muscle of spine and ectopic bone formation of the implanted gel in vivo was detected by hematoxylin and eosin stain (HE). PTMC11-F127-PTMC11 hydrogel with concentration more than 20 percent showed sustained slow release for one month after the initial burst release. Bone trabeculae surround the P24/ PTMC11-F127-PTMC11 hydrogel was shown at the end of six weeks by hematoxylin and eosin stain. These results indicated that encapsulated bone morphogenetic protein (BMP-2) derived peptide P24 remained viable in vivo, thus suggesting the potential of PTMC11-F127-PT- MC11 composite hydrogels as part of a novel strategy for localized delivery of bioactive molecules.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Ostéogenèse
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Peptides
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Pharmacologie
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Prothèses et implants
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Matériaux biocompatibles
/
Os et tissu osseux
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Chimie
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Systèmes de délivrance de médicaments
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Protéines morphogénétiques osseuses
/
Hydrogels
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Biomedical Engineering
Année:
2014
Type:
Article