Nimodipine modulates Bcl-2 and Bax mRNA expression after cerebral ischemia / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 170-172, 2004.
Article
Dans En
| WPRIM
| ID: wpr-236582
Responsable en Bibliothèque :
WPRO
ABSTRACT
In order to explore whether the member of Bcl-2 gene family, for example, Bcl-2 and Bax, are induced after cerebral ischemia, and whether expression of genes can be modulated by calcium-antagonist, the rat cerebral ischemic models were made by occluding left middle cerebral artery. The expression of Bcl-2 and Bax mRNA was measured by RT-PCR method. After middle cerebral artery occlusion (MCAO), the expression of both Bcl-2 and Bax mRNA were induced. Level of Bcl-2 mRNA increased steadily and level of Bax mRNA increased gradually at first, reached a peak after 24 h, then decreased slowly. After administration of nimodipine, Bcl-2 mRNA was up-regulated in the hippocampus 6 and 24 h after ischemia, while Bax mRNA was down-regulated 6 and 24 h after ischemia. Focal cerebral ischemia can induce proto-oncogenes to express, which was associated with apoptosis. Calcium-antagonist can up-regulate Bcl-2 mRNA and down-regulate Bax mRNA. The increased ratio of Bcl-2 and Bax mRNA may contribute to the anti-apoptic effect of nimodipine. The study indicates that pharmacological modulation of Bcl-2 family member expression could become a new strategy to manage neuronal damage.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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ARN messager
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Inhibiteurs des canaux calciques
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Nimodipine
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Encéphalopathie ischémique
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Rat Wistar
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Apoptose
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Protéines proto-oncogènes c-bcl-2
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Protéine Bax
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Génétique
Limites du sujet:
Animals
langue:
En
Texte intégral:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Année:
2004
Type:
Article