Analyses of Streptomyces coelicolor inner membrane proteome by multidimentional protein identification technology / 生物工程学报
Chinese Journal of Biotechnology
; (12): 814-819, 2005.
Article
Dans Zh
| WPRIM
| ID: wpr-237068
Responsable en Bibliothèque :
WPRO
ABSTRACT
Streptomyces coelicolor is the model species among streptomycetes. Until now, proteomic analyses of S. coelicolor have been conducted using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry method, few integral membrane proteins were identified due to the hydrophobic and low-abundance nature of these proteins. In this work, 154 possible inner membrane proteins from S. coelicolor were identified using high pH-proteinase K sample preparation method and multidimensional protein identification technology, among them 44 are integral membrane proteins containing at least one transmembrane domain, most peptides and their corresponding proteins were identified experimentally for the first time.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Spectrométrie de masse
/
Protéines bactériennes
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Membrane cellulaire
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Chimie
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Génome bactérien
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Protéome
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Streptomyces coelicolor
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Génétique
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Protéines membranaires
/
Méthodes
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2005
Type:
Article