Determination of aflatoxin B1, B2, G1, G2 in Ben Lamge granules by HPLC-FLD after multi-pretreatment clean-up / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3780-3785, 2015.
Article
Dans Zh
| WPRIM
| ID: wpr-237731
Responsable en Bibliothèque :
WPRO
ABSTRACT
To establish a multi-pretreatment method for the determination of aflatoxin B1, B2, G1, G2 in Chinese patent medicines, aflatoxins were analyzed by high performance liquid chromatography-fluorescence detector with post-column derivatization, after the multi-pretreatment of samples. The results showed that after the samples extracted with MeOH-H2O, dehydrated by anhydrous magnesium sulphate and sodium chloride, and finally purified by neutral alumina, the impurity interference of different sources in Chinese patent medicines matrix can be effectively removed, and the main peak can be nicely separated from the impurity peak. The detection limits were 0.25, 0.25, 0.50, 0.25 μg x L(-1) for AFB1, AFB2, AFG1, AFG2, respectively. The quantification limits were 1.00, 0.50, 1.00, 0.50 μg x L(-1), respectively. Aflatoxin B1, G1 showed a good linear relationship at a range of 1.0-50 μg x L(-1), aflatoxin B2, G2 at a range of 0.5-12.5 μg x L(-1) (R2 > 0.99). The average recovery was 80.40% - 108.6%. The present method is simple, reproducible with the reasonable recoveries and can be applied for the determination of aflatoxins in Chinese patent medicines.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Médicaments issus de plantes chinoises
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Contamination de médicament
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Chromatographie en phase liquide à haute performance
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Aflatoxines
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Formes posologiques
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Méthodes
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2015
Type:
Article