The role of caveolae in shear stress-induced endothelial nitric-oxide synthase activation / 生物医学工程学杂志
J. biomed. eng
; Sheng wu yi xue gong cheng xue za zhi;(6): 1020-1023, 2005.
Article
de Zh
| WPRIM
| ID: wpr-238288
Bibliothèque responsable:
WPRO
ABSTRACT
This article deals with the influence of shear stress on endothelial NO synthesis, and the role of caveolae in shear stress-induced eNOS activation. Human umbilical vascular endothelial cells (HUVEC) were cultured and exposed to different levels of laminal shear stress and Filipin, the perfused cultures were collected, and NO(2-)/NO(3-) was detected using nitrate reduction method. The structure of caveolae was observed through transmission electron microscopy (TEM). The level of NO(2-)-/NO(3-) was found to increase with the elevation of shear stress level (P < 0.01). It was the highest at 1.5 N/m2. After treatment with Filipin, the level of NO produced by HUVEC decreased significantly (P < 0.01), but after recovery and shear without Filipin, the level of NO synthesis bounded back (P < 0.01). It was then concluded that shear stress can induce endothelial NO synthesis and caveolae plays a key role in shear stress-induced eNOS activation.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
/
Physiologie
/
Veines ombilicales
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Endothélium vasculaire
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Cellules cultivées
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Filipine
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Cavéoles
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Biologie cellulaire
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Résistance au cisaillement
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Nitric oxide synthase type III
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
J. biomed. eng
/
Sheng wu yi xue gong cheng xue za zhi
Année:
2005
Type:
Article