Effect of microparticles on echinocandin B production by Aspergillus nidulans / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1082-1088, 2015.
Article
Dans Zh
| WPRIM
| ID: wpr-240594
Responsable en Bibliothèque :
WPRO
ABSTRACT
Anidulafungin is an effective antifungal medicine, which can inhibit activities of candida in vitro and in vivo. Echinocandin B (ECB) is the key precursor of Anidulafungin, thus the price and market prospect of Anidulafungin is directly due to the fermentation titer of ECB. In this study, Aspergillus nidulans was used for ECB fermentation, and the influence of adding microparticles on ECB fermentation was studied, such as talcum powder, Al2O3, and glass beads. The particle size and concentration were the key factors for mycelium morphology and ECB production, and ECB production could reach 1 262.9 mg/L and 1 344.1 mg/L by adding talcum powder of 20 g/L (d50 = 14.2 μm) and 7 glass beads (6 mm), an increase by 33.2% and 41.7%, respectively. The results indicated that the mycelium morphology of filamentous microorganisms and the product yield of fermentation could be improved by adding microparticles remarkably, and it provide an important method for the fermentative optimization of filamentous microorganisms.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Aspergillus nidulans
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Microbiologie industrielle
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Protéines fongiques
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Échinocandines
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Fermentation
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Métabolisme
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Méthodes
/
Antifongiques
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2015
Type:
Article