Effects of knockout of 2,3-butanediol synthesis key enzyme genes on 1,3-propandediol production in Klebsiella pneumoniae / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1290-1300, 2013.
Article
Dans Zh
| WPRIM
| ID: wpr-242481
Responsable en Bibliothèque :
WPRO
ABSTRACT
2,3-butanediol (2,3-BD) is a major byproduct of 1,3-propandediol (1,3-PDO) fermentation by Klebsiella pneumoniae. To decrease the formation of 2,3-BD, the budC and budA gene, coding two key enzymes of 2,3-BD synthetic pathway in K. pneumoniae, were knocked out using Red recombination technology. The growth of the two mutants were suppressed in different level. The budC deficient strain fermentation results showed that 1,3-PDO concentration increased to 110% and 2,3-butanediol concentration dropped to 70% of the parent strain. However, the budA deficient strain did not produce 1,3-PDO and 2,3-BD, and the final titer of lactic acid, succinic acid, ethanol and acetic acid increased remarkably compared with the parent strain. Further analysis of budC deficient strain fermentation inferred that K. pneumoniae possessed the 2,3-BD cycle as a replenishment pathway. The consequence provided a new evidence for reforming low-byproduct K. pneumoniae.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Acetolactate synthase
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Propylène glycols
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Protéines bactériennes
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Butylène glycols
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Carboxy-lyases
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Techniques de knock-out de gènes
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Génétique
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Glycérol
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Klebsiella pneumoniae
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Métabolisme
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2013
Type:
Article