Application study on multicolor combinational probe coding real-time PCR in detection of foodborne pathogens / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 210-214, 2009.
Article
de Zh
| WPRIM
| ID: wpr-242665
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the detection limit of multicolor combinational probe coding real-time PCR (MCPC-PCR) in detection of Salmonella and Staphylococcus aureus suspended in the food samples, and to apply MCPC-PCR to detect the samples of food poisoning.</p><p><b>METHODS</b>Series concentration of bacterium suspension (10(1) - 10(9) CFU/ml) was prepared by using 22 simulated samples including fresh meat and cakes and then MCPC-PCR was applied to detect Salmonella and Staphylococcus aureus in 22 samples. Enrichment broth of 101 frozen samples and 5 early patients' anal swabs in food poisoning cases were detected after the DNA samples were extracted.</p><p><b>RESULTS</b>The limits of MCPC-PCR assay in detecting Salmonella and Staphylococcus aureus were about 10(2) copies/test; 101 frozen enrichment broth of samples in food poisoning cases were detected by MCPC-PCR assay, of 23 positive samples, 18 were confirmed by bacteriology techniques; 96 samples detected by MCPC-PCR and bacteriology techniques had the same results, and the coincidence rate was 95.05%. Anal swabs, collected from 5 of early patients in a food poisoning case gave a clue to be Vibrio parahaemolyticus by MCPC-PCR assay and then were perfectly consistent with bacteriology assay.</p><p><b>CONCLUSION</b>As a method of high sensitivity and good specificity, MCPC-PCR assay can quickly and conveniently detect multiple pathogens existing in food samples, therefore we recommend it to be used in rapidly screening or simultaneous detection of food-borne diseases.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Salmonella
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Staphylococcus aureus
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Données de séquences moléculaires
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Contamination des aliments
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Réaction de polymérisation en chaîne
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Techniques bactériologiques
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Techniques de sonde moléculaire
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Sensibilité et spécificité
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Microbiologie alimentaire
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Génétique
Type d'étude:
Diagnostic_studies
langue:
Zh
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2009
Type:
Article