Study on mismatch repair genes of chronic myeloid leukemia / 中华血液学杂志
Chinese Journal of Hematology
; (12): 103-106, 2006.
Article
Dans Zh
| WPRIM
| ID: wpr-243986
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and regulation mechanism of mismatch repair (MMR) genes in chronic myeloid leukemia (CML).</p><p><b>METHODS</b>Expression of MMR genes hMSH2, hMSH3, hMSH6, hMLH1 and hPMS2 mRNAs in 62 CML patients and K562 cell line were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Expression of bcr-abl mRNA and MMR genes mRNA were detected by RT-PCR in 26 CML patients with allogeneic peripheral blood stem cell transplantation (allo-PBSCT) and 4 CML patients on imatinib treatment. Expression of bcr-abl mRNA was detected by RT-PCR and tyrosine phosphorylation of BCR-ABL fusion protein by Western blot.</p><p><b>RESULTS</b>Expression of hMSH2, hMSH3 and hMLH1 mRNA was significantly lower in CML and K562 cells than in normal control (P < 0.05). In 26 CML with allo-PBSCT and 4 CML patients on imatinib treatment, expressions of hMSH2, hMSH3 and hMLH1 mRNA was enhanced while expression of bcr-abl mRNA decreased. In CML MNC after imatinib treatment and in K562 cells, expression of hMSH2, hMSH3 and hMLH1 mRNA was enhanced while tyrosine phosphorylation of BCR-ABL fusion protein decreased.</p><p><b>CONCLUSION</b>Expressions of hMSH2, hMSH3 and hMLH1 mRNA were down-regulated by bcr-abl fusion gene.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Pipérazines
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Pyrimidines
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Benzamides
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ARN messager
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Leucémie myéloïde chronique BCR-ABL positive
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Expression des gènes
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Régulation de l'expression des gènes tumoraux
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Protéines de fusion bcr-abl
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Cellules K562
Limites du sujet:
Adult
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Aged
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Female
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Humans
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Male
langue:
Zh
Texte intégral:
Chinese Journal of Hematology
Année:
2006
Type:
Article