Functional study of mdr1 and GSTpi expression reversed by hairpin siRNA in K562/A02 cell line / 中华血液学杂志
Chinese Journal of Hematology
; (12): 17-20, 2006.
Article
de Zh
| WPRIM
| ID: wpr-244000
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of hairpin small interference RNA (shRNA) on mdr1 and GSTpi protein expression in multidrug resistance human leukemia cell line K562/A02.</p><p><b>METHOD</b>The shRNAs were synthesized targeting the coding region sequences of mdr1 (79 - 99 nt) and GSTpi (308 - 327 nt) respectively, and cloned to plasmid pSilencer2.1-U6 neo. The cloned products pSilence mdr1 and pSilence GSTpi were transfected into K562/A02 cells. Western blot and immunofluorescence analysis were used to detect the effectiveness and the specificity of the gene silence. 50% inhibition concentration (IC(50)) of doxorubicin (ADM) on K562/A02 cells was determined by MTT method.</p><p><b>RESULT</b>pSilence mdr1 and pSilence GSTpi reduced the expression of P-gp and GSTpi protein from 0.75 +/- 0.02 and 0.54 +/- 0.02 to 0.48 +/- 0.05 and 0.39 +/- 0.02 (P < 0.01) respectively, with no effect on alpha-tubulin expression in comparison with the mock treatment. Transfection of pSilence lamin A/C into K562/A02 decreased lamin A/C expression but had no effect on the expression of P-gp and GSTpi. Immunofluorescence assay also showed that shRNAs significantly reduced the P-gp and GSTpi positive cells from (71.25 +/- 9.65)% and (81.25 +/- 6.49)% to (35.25 +/- 5.97)% and (41.25 +/- 4.43)% (P < 0.01), respectively, compared with the mock treatment. The resistance indexes after transfection were decreased to 8 (pSilence mdr1) and 10 (pSilence GSTpi) respectively from 23 (mock transfection) (P < 0.01).</p><p><b>CONCLUSION</b>The shRNA could effectively and specifically reverse the multidrug resistance on K562/A02 cell line.</p>
Texte intégral:
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Indice:
WPRIM
Sujet Principal:
ARN messager
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Transfection
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Glycoprotéine P
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Multirésistance aux médicaments
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Résistance aux médicaments antinéoplasiques
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Cellules K562
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Petit ARN interférent
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Interférence par ARN
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Glutathione S-transferase pi
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Génétique
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Hematology
Année:
2006
Type:
Article