Methylation status of IEX-1 gene promotor CpG island in malignant hematopoietic cell lines / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 473-476, 2011.
Article
de Zh
| WPRIM
| ID: wpr-244899
Bibliothèque responsable:
WPRO
ABSTRACT
This study was to purposed to explore the methylation status changes of IEX-1 gene promoter CpG island and its relevance with occurrence of hematologic malignancies. The methylation status of IEX-1 gene promoter CpG island in 9 NB4, HL-60 U937, Raji, CA46, Jurkat, K562, CEM and Molt4 hematologic malignant cell lines was detected by using methylation-specific PCR, the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells treated by M. sssI enzyme and the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells untreated were used as positive and negative controls respectively. The results showed that the hypermethylation of IEX-1 gene promoter CpG islands was detected in NB4, Molt4 and Raji cell lines, as well as in normal peripheral blood mononuclear cells treated by M. sssl enzyme; the partial methylation status was found in CA46, CEM, U937, K562, HL-60 and Jurkat cell lines; the unmethylation status was observed in untreated normal peripheral blood mononuclear cells. It is concluded that the changes of methylation status of gene IEX-1 promoter CpG island correlates with hematologic malignancies to a certain extent.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Agranulocytes
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Régulation de l'expression des gènes tumoraux
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Régions promotrices (génétique)
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Ilots CpG
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Tumeurs hématologiques
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Méthylation de l'ADN
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Lignée cellulaire tumorale
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Protéines régulatrices de l'apoptose
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Génétique
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2011
Type:
Article