Isolation of mesenchymal stem cells from bone marrow filters by primary explant culture / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 459-463, 2011.
Article
de Zh
| WPRIM
| ID: wpr-244902
Bibliothèque responsable:
WPRO
ABSTRACT
This study was aimed to investigate whether mesenchymal stem cells (MSC) can be isolated from bone marrow filters which have always been discarded. The bone marrow (BM) particles from BM filters of 2 healthy donors were cultivated by primary explant culture. After expansion, the number of MSC was counted and their immunophenotype and differentiation potential were detected. The results indicated that many MSC were found in bone marrow particles from filters, and nearly 10(7) MSC were obtained at 3 passages of expansion. They not only possessed the characteristics of morphology and immunophenotype of MSC, but also could differentiate into osteoblasts, chondrocytes and adipocytes. In conclusion, a large amount of MSC can be obtained from BM filters if the BM particles were cultivated by primary explant culture.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Cellules de la moelle osseuse
/
Différenciation cellulaire
/
Séparation cellulaire
/
Cellules cultivées
/
Biologie cellulaire
/
Techniques de culture de tissus
/
Cellules souches mésenchymateuses
/
Méthodes
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2011
Type:
Article