A cyclotide against influenza A H1N1 virus from Viola yedoensis / 药学学报
Yao Xue Xue Bao
; (12): 905-912, 2014.
Article
de Zh
| WPRIM
| ID: wpr-244996
Bibliothèque responsable:
WPRO
ABSTRACT
Three cyclotides were isolated from the whole plant of Viola yedoensis in this study. The two, vary peptide E and cycloviolacin Y5, were previously reported, and a novel cycloviolacin VY1 was characterized according to the interpretation of MS/MS fragmentation of peptides which were produced from the reduced and alkylated parent peptide with the digestion of Endo Lys-C, trypsin and chymotrypsin, separately. The stability of remarkable resistance to proteolytic degradation by trypsin and chymotrypsin, and that of thermal denaturation was confirmed again. Besides, the IC50 value of cycloviolacin VY1 against influenza A H1N1 virus was (2.27 +/- 0.20) microg x mL(-1). It is the first cyclotide reported with anti-influenza A H1N1 virus activity in vitro assay.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Antiviraux
/
Pharmacologie
/
Chimie
/
Viola
/
Cyclotides
/
Sous-type H1N1 du virus de la grippe A
/
Spectrométrie de masse en tandem
langue:
Zh
Texte intégral:
Yao Xue Xue Bao
Année:
2014
Type:
Article