Differential transcription of Bcl-2 and Bax through the cell cycle in prostate cancer cell line / 中华外科杂志
Chinese Journal of Surgery
; (12): 768-771, 2008.
Article
de Zh
| WPRIM
| ID: wpr-245532
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differential expression of apoptosis associated gene Bcl-2 and Bax through cell cycle and its possible clinical meaning.</p><p><b>METHODS</b>The prostate cancer cell line PC-3 was synchronized in M, G1, S and G2 phase using modified thymine deoxyriboside blockage and high pressure N2O technique. The efficiency of synchronization was detected by flow-cytometry. RT-PCR and Western blot methods were used to examine the expression of Bcl-2 and Bax in mRNA and protein level.</p><p><b>RESULTS</b>The synchronized rate of M, G1, S and G2 phase were 92.1%, 87.0%, 80.2% and 75.9% respectively. Bcl-2 was constitutively expressed through the cell cycle, but both the mRNA and protein expression level of Bcl-2 were very high in the G1 phase, dramatically decreased in M, S and G2 phase. The expression level of Bax had no change through the cell cycle.</p><p><b>CONCLUSIONS</b>Cell cycle could influence the expression level of Bcl-2 significantly but not Bax, these might have some clinical relevance.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
/
Tumeurs de la prostate
/
ARN messager
/
Expression des gènes
/
Cycle cellulaire
/
Protéines proto-oncogènes c-bcl-2
/
Lignée cellulaire tumorale
/
Protéine Bax
/
Génétique
/
Métabolisme
Limites du sujet:
Humans
/
Male
langue:
Zh
Texte intégral:
Chinese Journal of Surgery
Année:
2008
Type:
Article