Optimizing expression of the capsid protein VP2 from human Bocavirus and establish it's seroepidemiology assying methord / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 18-21, 2012.
Article
de Zh
| WPRIM
| ID: wpr-246194
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To obtain sufficient recombinant VP2 protein of human Bocavirus and establish it's seroepidemiology assying metbord. METHORD: Tbe capsid protein VP2 DNA genes of HBoV1 and 2 were optimized in accordance with tbe usage of the favorite codons in K coil so as to enhance its protein expression in prokaryotic expressing system. The protein was purified by Ni-NTA column, and its antigenicity was determined by Western Blot. Then establish ELISA to detect the specific anti-VP2 IgG antibodies against HBoV1 and 2 in healthy children aged 3-6 years in Nanjing, China.</p><p><b>RESULTS</b>The recombinant protein 6 x His-VP2 was produced in a larger quantity at 25 degrees C induced by IPTG (1 mmol/L) over night and purified by Ni-NTA column. Seropositive rates of HBoV1 and 2 were 62.2% and 55.5% and their mixed seropositivity was 37%.</p><p><b>CONCLUSION</b>The optimizing expression of the capsid protein VP2 from human Bocavirus constructed successfully and get a high yield under certain conditions. The established ELISA could be used to further analyze seroepidemiology of HBoV in China.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Test ELISA
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Études séroépidémiologiques
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Réaction de polymérisation en chaîne
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Protéines de capside
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Bocavirus humain
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Génétique
Limites du sujet:
Child
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Child, preschool
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Female
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Humans
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Male
langue:
Zh
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2012
Type:
Article