Lateral flow immunoassay for quantitative detection of ractopamine in swine urine / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 134-137, 2014.
Article
de En
| WPRIM
| ID: wpr-247072
Bibliothèque responsable:
WPRO
ABSTRACT
A strip reader based lateral flow immunoassay (LFIA) was established for the rapid and quantitative detection of ractopamine (RAC) in swine urine. The ratio of the optical densities (ODs) of the test line (AT) to that of the control line (AC) was used to effectively minimize interference among strips and sample variations. The linear range for the quantitative detection of RAC was 0.2 ng/mL to 3.5 ng/mL with a median inhibitory concentration (IC50) of 0.59 ± 0.06 ng/mL. The limit of detection (LOD) of the LFIA was 0.13 ng/mL. The intra-assay recovery rates were 92.97%, 97.25%, and 107.41%, whereas the inter-assay rates were 80.07%, 108.17%, and 93.7%, respectively.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phénéthylamines
/
Bandelettes réactives
/
Suidae
/
Urine
/
Dosage immunologique
Type d'étude:
Diagnostic_studies
Limites du sujet:
Animals
langue:
En
Texte intégral:
Biomedical and Environmental Sciences
Année:
2014
Type:
Article