Rapid detection of chromosomes X and Y using dual-color primed in situ labeling technique / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 477-478, 2007.
Article
de Zh
| WPRIM
| ID: wpr-247287
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a rapid method for detection of chromosomes, and to present a feasible method for rapid detection of the interphase nuclei of uncultured amniocytes using modified dual-color primed in situ labeling (PRINS) technique.</p><p><b>METHODS</b>Chromosomes X and Y were detected and analyzed by the modified dual-color PRINS in 205 amniotic fluid samples.</p><p><b>RESULTS</b>The specific chromosomes were obtained on both metaphase and interphase nuclei. In more than 98% of the samples PRINS reactions with primers X and Y were successfully induced. One sample was properly identified and correctly scored as 47, XXY.</p><p><b>CONCLUSION</b>The results suggest that PRINS technique is rapid, simple and cost-effective. It is also sensitive and specific, and thus useful for rapid detection of chromosome abnormalities.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Reproductibilité des résultats
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Sensibilité et spécificité
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Synthèse in situ amorcée
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Maladies chromosomiques
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Chromosomes X humains
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Chromosomes Y humains
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Diagnostic
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Génétique
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Méthodes
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites du sujet:
Adolescent
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Adult
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Female
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Humans
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Pregnancy
langue:
Zh
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2007
Type:
Article