Cultivation and karyotype analysis of the human embryonic stem cells HUES4 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 275-278, 2007.
Article
de Zh
| WPRIM
| ID: wpr-247336
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a protocol for culturing of human embryonic stem cells (HUES4) without any animal-derived feeder cells and to investigate the karyotype stabilities of HUES4 cells after long-term cultivation.</p><p><b>METHODS</b>HUES4 cells were cultured on mitomycin C treated MEFs or human foreskin fibroblast feeder cells. The pluripotency of the ES cells was analyzed by immunocytochemistry staining to detect the expression of pluripotent marker, karyotype of the ES cells at passage 27, 34, 41, 44 and short tandem repeat (STR) at passage 27 were analyzed.</p><p><b>RESULTS</b>The HUES4 cells cultured on human feeder cells were positive for alkaline phosphatase activity, SSEA-4, TRA-1-60 and TRA-1-81 staining, but negative for SSEA-1. Analysis of karyotype at different passages suggested an abnormal karyotype 46, XY, t(9;15)(q22;q26) mosaicism occurred in HUES4, and the ratios of abnormal increased with passage.</p><p><b>CONCLUSION</b>HUES4 could be cultured without animal-derived feeder cells and the incidence of abnormal karyotype might be increased with long-term culture.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Polymorphisme génétique
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Immunohistochimie
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Lignée cellulaire
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Répétitions microsatellites
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Techniques de culture cellulaire
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Biologie cellulaire
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Prolifération cellulaire
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Prépuce
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Cellules souches embryonnaires
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Fibroblastes
Limites du sujet:
Humans
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Male
langue:
Zh
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2007
Type:
Article