An experimental study on recombinant adenovirus p53 transfected in oral dysplastic epithelial cells / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 122-125, 2009.
Article
Dans Zh
| WPRIM
| ID: wpr-248292
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate and evaluate the appropriate virus titer and transfection efficiency of recombinant adenovirus p53 into the oral dysplastic epithelial cells (POE-9n) and provide reference for oral precancerosis research.</p><p><b>METHODS</b>The transfection sensitivity of adenovirus into oral dysplastic epithelial cells was evaluated by the recombinant adenovirus p53 containing green fluorescent protein (rAd-GFP). Different titre rAd -p53 was transfected into oral dysplastic epithelial cells to evaluate the effects of rAd-p53 on cell proliferation inhibition by MIT assay. The expression of exogenous p53 gene in POE-9n cells was detected by immunocytochemistry.</p><p><b>RESULTS</b>More than 95% POE-9n cells were transfected by rAd-GFP with MOI from 100 to 500 and there was no statistical difference between different MOI values (r=-0.124, P>0.05). It was found that rAd-p53 had significant inhibition effects on POE-9n cell proliferation with MOI from 100 to 500, and there were no significant differences at 96 h and 120 h after the transfection on cell proliferation inhibition (P>0.05). P53 protein was well expressed in rAd-p53 transfected POE-9n cells.</p><p><b>CONCLUSION</b>Exogenous p53 can be successfully transfected into POE-9n cells by rAd-p53 and the virus titer of MOI 100 was high enough to ensure efficient transfection.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Transfection
/
Adenoviridae
/
Gènes p53
/
Protéines à fluorescence verte
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Prolifération cellulaire
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Cellules épithéliales
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Vecteurs génétiques
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
West China Journal of Stomatology
Année:
2009
Type:
Article