Application of interphase fluorescence in situ hybridization in the diagnosis of lymphoma / 中华病理学杂志
Chinese Journal of Pathology
; (12): 733-738, 2009.
Article
Dans Zh
| WPRIM
| ID: wpr-249052
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility of interphase FISH in the routine clinicopathological practice and its values in the differential diagnosis of lymphomas.</p><p><b>METHODS</b>A total of 74 fresh tissue samples clinically suspicious of lymphoma were investigated by FISH using three probes including IgH/bcl-2, IgH/CCND1 and API2/MALT1, corresponding the translocation t(14;18), t(11;14) and t(11;18) respectively. The results of FISH were analyzed and compared with the histopathologic diagnosis.</p><p><b>RESULTS</b>Histological evaluation eventually confirmed that there were 62 cases of lymphoma and 12 cases of reactive lymphoid processes. The translocations were detected in 7 cases in 62 cases of lymphoma 3 demonstrated t(14;18) including 2 cases of follicular lymphomas and 1 nodular sclerosing Hodgkin lymphoma. Four cases had t(11;14) including mantle cell lymphoma (2 cases), follicular lymphoma (1 case) and small cell lymphoma (1 case). A lymphoid hyperplasia case showed detectable t(14;18). All 25 cases of DLBCL showed no evidence of t(14;18). Amplification or loss of regional genes was seen more often in malignant than in the benign cases.</p><p><b>CONCLUSION</b>Interphase FISH offers useful ancillary technology that plays an important role in differential diagnosis and classification of lymphoma.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Translocation génétique
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Chromosomes humains de la paire 11
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Chromosomes humains de la paire 14
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Chromosomes humains de la paire 18
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Lymphome malin non hodgkinien
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Maladie de Hodgkin
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Leucémie chronique lymphocytaire à cellules B
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Lymphome folliculaire
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Lymphome B diffus à grandes cellules
Type d'étude:
Diagnostic_studies
Limites du sujet:
Adolescent
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Adult
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Aged
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Aged80
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Child
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Female
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Humans
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Male
langue:
Zh
Texte intégral:
Chinese Journal of Pathology
Année:
2009
Type:
Article