Influence of CO2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 650-655, 2005.
Article
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| ID: wpr-249155
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ABSTRACT
<p><b>OBJECT</b>The authors studied the influence of CO(2) pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation.</p><p><b>METHOD</b>They set up a simulation of pneumoperitoneum under different CO(2) pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated.</p><p><b>RESULT</b>When the pressure of CO(2) pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5, 178.0, 181.6 nmol/(g.min) and PP2a was 4158.3, 4066.9, 3984.0, 3878.5 nmol/(g.min) respectively. After 1 week, the cancer cells concentration was 2.15 x 10(5), 2.03 x 10(5), 2.20 x 10(5), 2.18 x 10(5) L(-1).</p><p><b>CONCLUSION</b>CO(2) pneumoperitoneum could promote acidosis in cancer cells, inducing the activation of protein kinase C and deactivation of protein phosphatase 2a, but it could not accelerate the mitosis rate of the cancer cells.</p>
Texte intégral:
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Indice:
WPRIM
Sujet Principal:
Pneumopéritoine artificiel
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Protéine kinase C
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Tumeurs du sein
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Dioxyde de carbone
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Transduction du signal
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Survie cellulaire
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Chimie
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Phosphoprotein Phosphatases
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Lignée cellulaire tumorale
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Prolifération cellulaire
Limites du sujet:
Animals
langue:
En
Texte intégral:
Journal of Zhejiang University. Science. B
Année:
2005
Type:
Article