Effect of recipient mouse splenocytes taken orally by donor mouse on GVHD after splenocyte transplantation / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 126-130, 2008.
Article
de Zh
| WPRIM
| ID: wpr-253367
Bibliothèque responsable:
WPRO
ABSTRACT
This study was aimed to explore whether the GVHD in mice can be ameliorated and the GVL effect in mice can be reserved by transfusion of lymphocytes of donors fed with recipient splenocytes effect. Male (DBA-2) mice (H-2(d)) as donors were fed with BALB/c splenocytes, DBA-2 splenocytes, bovine serum albumin, or regular chow, every other day. Induction of tolerance was assessed by a mixed lymphocyte reaction (MLR). Female (BALB/c) mice (H-2(d)) as recipients received total body irradiation (TBI) of 6.0 Gy ((60)Cogamma-ray) followed by inoculation of 3 x 10(3) P388 mouse leukemia cells on the same day. Subsequently, tail vein injection of 2 x 10(7) splenocytes supplied by DBA-2 was undertaken. Control groups were fed identically without leukemia cell inoculation. The results showed that GVHD was significantly ameliorated and CD4(+)/CD8(+) ratio increased in recipient-mice transplanted with splenocytes of tolerated donors, compared with control animals. There was no significant difference in survival rate between different groups of recipients inoculated with leukemia cell. It is concluded that the peroral recipient-mouse splenocytes can ameliorate GVHD without hampering effect on GVL.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
/
Rate
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Thérapeutique
/
Lymphocytes
/
Leucémie P388
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Extrait cellulaire
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Adjuvants immunologiques
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Irradiation corporelle totale
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Transplantation cellulaire
/
Réaction du greffon contre la leucémie
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2008
Type:
Article