Effects of Newcastle disease virus on the mitochondria of human gastric carcinoma BGC-823 cells / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 198-200, 2008.
Article
de Zh
| WPRIM
| ID: wpr-254105
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore changes in structure and function of the mitochondria of human gastric carcinoma BGC-823 cells after Newcastle disease virus (NDV) infection.</p><p><b>METHODS</b>Electron microscopy was applied to observe the structure of mitochondria; Rhodamine 123 staining was used to determine the mitochondrial membrane potential; the activity of Na(+)-K(+)-ATPase and Ca(2+)-ATPase were also determined and the release of cytochrome C was detected by Western blotting.</p><p><b>RESULTS</b>The structure of mitochondria in the tumor cells infected with NDV changed distinctly. In the infected group the activity of mitochondrial Na(+)-K(+)-ATPase and Ca(2+)-ATPase significantly declined (P < 0.01), and compared with control cells, mitochondrial trans-membrane potential was decreased. NDV infection induced the decrease of cytochrome C levels.</p><p><b>CONCLUSION</b>The effects of NDV infection on the structure and functions of mitochondria of human gastric carcinoma BGC-823 cells might play a role in the oncolysis of NDV.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Physiologie
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Tumeurs de l'estomac
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Virologie
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Virus de la maladie de Newcastle
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Carcinomes
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Sodium-Potassium-Exchanging ATPase
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Lignée cellulaire tumorale
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Cytochromes c
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Potentiel de membrane mitochondriale
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Métabolisme
Limites du sujet:
Animals
/
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2008
Type:
Article