The role of hepatitis B virus X gene and p53 on hepatocellular carcinoma cell growth / 中华病理学杂志
Chinese Journal of Pathology
; (12): 43-47, 2003.
Article
Dans Zh
| WPRIM
| ID: wpr-255358
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of hepatitis B virus X gene and p53 on hepatocellular growth.</p><p><b>METHODS</b>Two kinds of plasmids containing sense and antisense human wild p53 gene respectively were constructed. SMMU-7721 cells were transfected with HBx, sense-wtp53 antisense-wtp53 separately or cotransfected with either HBx and sense-wtp53 or HBx and antisense-wtp53. Flow cytometry was adopted to measure the apoptosis rates and the effects of HBx on cell cycle progression. The activity of p21(Waf1) promoter-luciferase construct was detected. Growth curves for SMMU-7721 stably transfected with pcDNA3 and pcDNA3HBx were analyzed.</p><p><b>RESULTS</b>After doxorubicin administration, HBx was noticed able to initiate apoptosis of the liver cells. The apoptosis rate was 5.32% in the pcDNA3 transfected and 12.66% in the pcDNA3HBx transfected groups respectively. HBx could also abrogate p53-mediated apoptosis. The apoptosis rate in groups transfected with pcDNA3, pcDNA3wtp53 and pcDNA3HBx + pcDNA3wtp53 was 5.32%, 11.72% and 4.67% respectively. In compared with the normal group, the number of cells in transiently HBx-expressed group and HBx-transfected group decreased 4.79% and 10.25% respectively. HBx inhibited the activity of p21(Waf1) promoter-luciferase constructed (P < 0.05) and promoted cell growth. The growth rate of HBx expression cells was faster.</p><p><b>CONCLUSION</b>Under DNA damage, HBx reduced expression of p21(Waf1) by repressing the activity of p53 protein, followed by disturbing the regulation of G(0)-G(1) cell cycle checkpoint, and promoted the growth rate of hepatoma cells.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Virologie
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Transfection
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Transactivateurs
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Division cellulaire
/
Virus de l'hépatite B
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Gènes p53
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Protéine p53 suppresseur de tumeur
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Apoptose
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Carcinome hépatocellulaire
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Pathology
Année:
2003
Type:
Article