Influence of betulinic acid on proliferation, migration, cell cycle and apoptosis of pancreatic cancer cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3056-3059, 2010.
Article
de Zh
| WPRIM
| ID: wpr-260688
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of betulinic acid (BA) on the proliferation, migration, apoptosis and cell cycle of pancreatic cancer cells (BxPC-3) in vitro and elucidate the underlying.</p><p><b>METHOD</b>The effect of BA on the proliferation of BxPC-3 was measured by using sulforhodamine B (SRB) assay. Migratory ability of BxPC3 cells were detected by wound healing assay, and the morphological change was observed with light microscope. The influence of BA on cell cycle of BxPC-3 cells was tested by flow cytometry (FCM). Apoptosis was analyzed by using Hochest33342-PI double staining. Western blot technologies were applied to detect the expression of Bcl-2 and Bax.</p><p><b>RESULT</b>BA exhibited significant cell proliferation and migration inhibition, as well as its potency of inducing apoptosis in BxPC-3 cells in vitro in a dose-dependent manner. The IC50 value for 72 h was 16.54 mg x L(-1). Cell migration was significantly inhibited at 5 mg x L(-1) of BA. Cells treated with BA showed increased cell population in G0 phase, with decreased G2/M phase population. The expression of Bax and Bcl-2 was up and down-regulated respectively in BA-treated BxPC-3 cells in a dose-dependent manner.</p><p><b>CONCLUSION</b>BA exerted potent effect on growth inhibition, G0 cell cycle arrest and induction of apoptosis in BxPC-3 cells in vitro, possibly associated with the down-regulation of Bcl-2 and up-regulation of Bax expression. The potent antitumor capacity of BA suggested that it could be a promising new anticancer agent in human pancreatic cancer treatment.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Tumeurs du pancréas
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Pharmacologie
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Triterpènes
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Médicaments issus de plantes chinoises
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Régulation de l'expression des gènes tumoraux
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Cycle cellulaire
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Mouvement cellulaire
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Apoptose
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Protéines proto-oncogènes c-bcl-2
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Lignée cellulaire tumorale
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2010
Type:
Article