Clinical value of interphase fluorescent in situ hybridization in diagnosis of core-binding factor acute myelocytic leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1156-1160, 2011.
Article
de Zh
| WPRIM
| ID: wpr-261910
Bibliothèque responsable:
WPRO
ABSTRACT
The purpose of this study was to evaluate the clinical value of interphase fluorescence in situ hybridization (I-FISH) in diagnosis of core-binding factor acute myelocytic leukemia (CBF AML). The cytogenetic characteristics in leukemia cells from 82 cases of AML-M(2) and 43 cases of AML-M(4)/M(5) were detected by using I-FISH with AML1-ETO double color double fusion probe and double color break point isolated gene probe CBFβ-MYH11, and the detected results were compared with results detected by conventional cytogenetic R banding technique (CC). The results indicated that AML1-ETO fusion gene was detected in 30.5% cases (25/82) by FISH, and t(8;21)(q22;q22) karyotypic aberrations was found in 28.0% cases (23/82) by CC method. Among 25 FISH positive cases, typical FISH positive signal pattern (1R1G2F) was displayed in 22 cases and atypical signal pattern (1R2G1F and 2R1G2F) was found in the other 3 cases. Among all 43 AML-M(4)/M(5) cases, the CBFβ-MYH11 fusion gene was detected in 23.3% cases (10/43) by FISH, which sensitivity was significant higher than that by CC method (2/43) (p < 0.05). It is concluded that some insufficiency of CC technique can be compensated by FISH, and combination of I-FISH with CC technique play a crucial role in diagnosis of CBF AML and in monitoring of minimal residual disease.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Leucémie aigüe myéloïde
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Hybridation fluorescente in situ
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Analyse cytogénétique
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Diagnostic
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Facteurs de transcription CBF
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Génétique
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Caryotypage
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Méthodes
Type d'étude:
Diagnostic_studies
Limites du sujet:
Adolescent
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Adult
/
Child
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Female
/
Humans
/
Male
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2011
Type:
Article