MIP-1α promotes the migration ability of Jurkat cell through human brain microvascular endothelial cell monolayer / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 35-39, 2014.
Article
de Zh
| WPRIM
| ID: wpr-264954
Bibliothèque responsable:
WPRO
ABSTRACT
This study was purposed to explore the mechanism of central nervous system (CNS) leukemia resulting from brain metastasis of human acute T-cell leukemia (T-ALL) cells and the role of MIP-1α in migration of Jurkat cells through human brain microvascular endothelial cells (HBMEC). The real-time PCR, siRNA test, transendothelial migration test, endothelial permeability assay and cell adhesion assay were used to detect MIP-1α expression, penetration and migration ability as well as adhesion capability respectively. The results showed that the MIP-1α expression in Jurkat cells was higher than that in normal T cells and CCRF-HSB2, CCRF-CEM , SUP-T1 cells. The MIP-1α secreted from Jurkat cells enhanced the ability of Jurkat cells to penetrate through HBMEC, the ability of Jurkat cells treated by MIP-1α siRNA to adhere to HBMEC and to migrate trans endothelial cells decreased. It is concluded that the MIP-1α secreted from Jurkat cells participates in process of penetrating the Jurkat cells through HBMEC monolayer.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
/
Tumeurs du cerveau
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Endothélium vasculaire
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Adhérence cellulaire
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Mouvement cellulaire
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Cellules Jurkat
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Cellules endothéliales
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Leucémie-lymphome lymphoblastique à précurseurs T
/
Chimiokine CCL3
/
Métabolisme
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2014
Type:
Article