Effect of microRNA-17-92 cluster on the biological characteristics of K562 cells and its mechanisms / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 20-24, 2014.
Article
de Zh
| WPRIM
| ID: wpr-264957
Bibliothèque responsable:
WPRO
ABSTRACT
The objective of this study was to explore the effects of microRNA-17-92 on the biological characteristics of K562 cells. The expression of miR-17-92 in K562 cells transfected with miRNA-17-92 mimic was detected by real time PCR. The effect of microRNA-17-92 on K562 cell proliferation was detected by CCK-8 method. Apoptosis of K562 cells was detected by Annexin V-PI labeling. Cell cycle distribution was determined by using flow cytometry. Western blot was performed to determine the protein levels of Crk. The results indicated that the transfection with miR-17-92 mimic increased expression of mature miR-17-92 in K562 cells. Compared with control group, cell proliferation and cell amount in S-phase of miR-17-92 mimic transfected group significantly increased, cell apoptosis decreased. The expression of signal connector protein Crk was greatly up-regulated in miR-17-92-mimic-transfected K562 cells. It is concluded that miR-17-92 can promote proliferation, inhibit apoptosis and regulate the cell cycle of K562 cells.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Transfection
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Leucémie myéloïde chronique BCR-ABL positive
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Régulation de l'expression des gènes dans la leucémie
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Cycle cellulaire
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Apoptose
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Cellules HL-60
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Cellules K562
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MicroARN
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Prolifération cellulaire
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Génétique
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2014
Type:
Article