Changes of sphingolipids profiles after ischemia-reperfusion injury in the rat liver / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 3025-3031, 2009.
Article
de En
| WPRIM
| ID: wpr-265965
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Hepatic ischemia-reperfusion (I/R) injury occurs in many clinical procedures. The molecular mechanisms responsible for hepatic I/R injury however remain unknown. Sphingolipids, in particular ceramide, play a role in stress and death receptor-induced hepatocellular death, contributing to the progression of several liver diseases including liver I/R injury. In order to further define the role of sphingolipids in hepatic I/R, systemic analysis of sphingolipids after reperfusion is necessary.</p><p><b>METHODS</b>We investigated the lipidomic changes of sphingolipids in a rat model of warm hepatic I/R injury, by delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry (DE MALDI-TOF-MS).</p><p><b>RESULTS</b>The total amounts of ceramide and sphingomyelin and the intensity of most kinds of sphingolipids, mainly sphingomyelin, significantly increased at 1 hour after reperfusion (P < 0.05) and reached peaks at 6 hours after reperfusion (P < 0.01) compared to controls. Six new forms of ceramide and sphingomyelins appeared 6 hours after reperfusion, they were (m/z) 537.8, 555.7, 567.7, 583.8, 683.5 and 731.4 respectively. A ceramide-monohexoside (m/z) 804.4 (CMH(d18:1C22:1+Na)(+)) also increased after reperfusion and correlated with extent of liver injury after reperfursion.</p><p><b>CONCLUSIONS</b>Three main forms of sphingolipids, ceramide, sphingomyelin and ceramide-monohexoside, are related to hepatic I/R injury and provide a new perspective in understanding the mechanisms responsible for hepatic I/R injury.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Sphingolipides
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Lésion d'ischémie-reperfusion
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Facteur de nécrose tumorale alpha
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Rat Sprague-Dawley
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Spectrométrie de masse MALDI
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RT-PCR
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Génétique
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Foie
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Métabolisme
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
langue:
En
Texte intégral:
Chinese Medical Journal
Année:
2009
Type:
Article