Characteristics of Yersinia enterocolitica genes isolated in Ningxia Hui autonomous region from 1997 to 2010 / 中华预防医学杂志
Zhonghua Yu Fang Yi Xue Za Zhi
; (12): 886-889, 2011.
Article
de Zh
| WPRIM
| ID: wpr-266079
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the distribution of virulent genes of Yersinia enterocolitica (Y. enterocolitica) in Ningxia Hui autonomous region and the characteristics of the molecular patterns of Y. enterocolitica.</p><p><b>METHODS</b>283 strains of Y. enterocolitica were isolated in Ningxia Hui autonomous region between year 1997 and 2010. The genes ail, ystA, ystB, yadA and virF were analyzed by PCR method; the chromosomal DNA of Y. enterocolitica was digested by restriction endonucleases NotI and processed by pulsed-field gel electrophoreses (PFGE); and then the cluster analysis were conducted by BioNumeric computer software towards the above results.</p><p><b>RESULTS</b>Of all, 209 strains of serotypes O:3 and O:9 Y.enterocolitica showed positive virulence of genes ail, ystA, yadA and virF; 97.6% (204/209) of which, the ystB virulence were negative. The virulence of all genes in serotype O:8 and serum-unclassified strains were negative. 9 out of 11 strains of serotype O:5 Y. enterocolitica showed negative virulence of the above five genes. By PFGE, according to the NotI Macrorestriction Map on chromosomal DNA, the 29 strains of serotype O:3 Y. enterocolitica were divided into 12 PFGE patterns, 2 of which were dominant patterns which could be found in over 5 strains; and the 180 strains of serotype O:9 Y. enterocolitica were divided into 13 patterns, 4 of which were dominant patterns which existed in over 10 strains; which were isolated individually from pigs and house mouse, pigs and dogs as well as pigs and wild rabbits.</p><p><b>CONCLUSION</b>Y.enterocolitica serotypes O:3 and O:9 were pathogenic in Ningxia, and serotype O:3 becomes predominant gradually. O:5, O:8 and serum-unclassified serotypes were non-pathogenic.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Toxines bactériennes
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Virulence
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Yersinia enterocolitica
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Yersinioses
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ADN bactérien
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Électrophorèse en champ pulsé
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Sus scrofa
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Gènes bactériens
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Génétique
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Méthodes
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Zhonghua Yu Fang Yi Xue Za Zhi
Année:
2011
Type:
Article