Autophagy is involved in 6-OHDA-induced dopaminergic cell death / 南方医科大学学报
Journal of Southern Medical University
; (12): 2649-2651, 2010.
Article
Dans Zh
| WPRIM
| ID: wpr-267717
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of autophagy in the death of dopaminergic neurons induced by 6-hydroxydopamine (6-OHDA).</p><p><b>METHODS</b>Rat models of Parkinson disease (PD) were established by stereotaxic administration of 6-OHDA (8 μg) into the unilateral substantia nigra par compact (SNpc). Autophagosomes in the SNpc were observed with transmission electron microscopy (TEM), and the expression of autophagy-related protein LC3 was determined with immunofluorescence (IF) assay.</p><p><b>RESULTS</b>Under TEM, the autophagosomes were found in the ipsilateral SNpc 6-24 h after 6-OHDA injection, which suggested the activation of autophagy. IF assay showed significantly increased LC3 expression in 6-OHDA-damaged TH-positive neurons as compared to the control group.</p><p><b>CONCLUSIONS</b>The increase of autophagosomes and activation of autophagy may play a role in dopaminergic neuron death induced by 6-OHDA.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Syndrome parkinsonien secondaire
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Pharmacologie
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Autophagie
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Phagosomes
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Substantia nigra
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Oxidopamine
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Mort cellulaire
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Rat Sprague-Dawley
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Biologie cellulaire
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Modèles animaux de maladie humaine
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2010
Type:
Article