Directional differentiation of murine CD117+ hemopoietic stem cells into immature dendritic cells and their identification / 南方医科大学学报
Journal of Southern Medical University
; (12): 450-453, 2007.
Article
de Zh
| WPRIM
| ID: wpr-268108
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a stable method for obtaining large quantity of highly purified immature dendritic cells (imDCs) in vitro, and identify the morphology, function and surface markers of the cells.</p><p><b>METHODS</b>CD117(+) hemopoietic stem cells (HSCs) were isolated and purified from the bone marrow of healthy C57 mice by magnetic affinity cell sorting. After cell expansion by treatment with stem cell factor (SCF) and interleukin-3 (IL-3), the HSCs were induced for directional differentiation into imDCs by treatment with GM-CSF, IL-4 and IL-10. The imDCs obtained were identified by morphological and functional observation under inverted microscope, scanning electron microscope and transmission electron microscope, followed by detection of the expressions of the surface markers using flow cytometry.</p><p><b>RESULTS</b>After 3, 5 and 7 days of culture in the presence of SCF+IL-3, the cells were expanded by 10.34-/+1.43, 22.65-/+2.71 and 54.39-/+3.08 folds, respectively. The HSCs were successfully induced to differentiate into imDCs with phagocytotic activity. The dendrites of the imDCs were short small, and appearing spinous. The expressions of surface markers were detected from the cells showing the phenotype of CD11c(+), I-A/I-E(low), CD40(-), CD80(-), CD86(-).</p><p><b>CONCLUSION</b>The method described allows steadily acquisition of large quanty of highly purified imDCs and of their effective identification in vitro.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Cellules dendritiques
/
Cellules souches hématopoïétiques
/
Microscopie électronique à balayage
/
Différenciation cellulaire
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Séparation cellulaire
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Cellules cultivées
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Protéines proto-oncogènes c-kit
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Techniques de culture cellulaire
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Biologie cellulaire
/
Microscopie électronique à transmission
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2007
Type:
Article