Ultrastructural and element spectrometric analysis of distraction osteogenesis for reconstruction of cleft palate in rhesus macaque model / 中华整形外科杂志
Chinese Journal of Plastic Surgery
; (6): 275-280, 2010.
Article
de Zh
| WPRIM
| ID: wpr-268690
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the ultrastructure and Ca/P element spectrometry of distraction osteogenesis (DO) for reconstruction of cleft palate (CP), so as to explore the osteogenesis and remodeling of new bone in situ.</p><p><b>METHODS</b>23 rhesus macaques were operated to establish animal models of CP. 2 monkeys didn't received DO as controls. The other 21 monkeys in experimental group underwent DO to correct both bony and soft tissue defects in palate. The distraction was performed at a rate of 0.8 mm/d, twice a day until the cleft was closed. After fixation for 1, 2, 4, 6, 8, 12, 24 weeks, every 3 animals were sacrificed to get the specimens at the distraction gap. The scanning electron microscopic study and Ca, P elements spectrometric analysis were adopted. There were also two unoperated animals as sham group.</p><p><b>RESULTS</b>After fixation for 1-2 weeks, the distraction gap was full of collagen fibers oriented along vector of distraction. Few trabeculae was seen at the margin area. After fixation for 4-6 weeks, active osteogenesis was presented with new formed bone trabeculae and abundant cellular component. After fixation for 8-12 weeks, the new formed bone became mature and couldn't distinguish from the normal bone. 24 weeks later, the bone between the distraction gap had a similar structure to the normal bone. Elements spectrometric analysis results indicated that in early stage of osteogenesis, the P and S peaks were relatively high while the Ca peak was much lower. During the late stage, the S peak was obviously decreased, and Ca/P ratio increased to normal level as in the empty control group.</p><p><b>CONCLUSIONS</b>The CP can be corrected by DO. The new bone between the distraction gap is formed and remodeled through intramembraneous osteogenesis.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Ostéogenèse
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Palais
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Anatomopathologie
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Chirurgie générale
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Microscopie électronique à balayage
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Fente palatine
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Ostéogenèse par distraction
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Modèles animaux de maladie humaine
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Macaca mulatta
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Métabolisme
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Plastic Surgery
Année:
2010
Type:
Article