Etiologic investigation of chronic granulomatous inflammation of hand by polymerase chain reaction and DNA sequencing / 中华病理学杂志
Chinese Journal of Pathology
; (12): 669-671, 2006.
Article
Dans Zh
| WPRIM
| ID: wpr-268870
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the causative pathogens in littoral hand infections which exhibited chronic granulomatous inflammation, the relationship between chronic granulomatous inflammation and mycobacteria and to discuss the prospects of PCR in clinical application for diagnosis of granulomatous inflammation.</p><p><b>METHOD</b>With 16S-rDNA as the target sequence, Nest-PCR was used to detect mycobacteria directly from 37 cases of chronic granulomatous inflammations, and identified them by gene sequencing.</p><p><b>RESULTS</b>Twenty-four of 37 cases were positive for mycobacteria by Nest-PCR, in which 17 were M.marinum, 1 M.chelonae, 2 M.avium, 2 M.kansasii, and 2 M.tubercular through gene sequencing.</p><p><b>CONCLUSIONS</b>Nest-PCR combining gene sequencing proved to be a liable and sensitive method to detect Non-tubercular mycobacteria (NTM) in fresh tissue. NTM is the major factor of hand specific chronic infections other than tubercular. Pathological changes are difficult to differentiate TB from NTM and bacterial evidence was necessary.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
ADN bactérien
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ARN ribosomique 16S
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Chimie
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Réaction de polymérisation en chaîne
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Maladie chronique
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Analyse de séquence d'ADN
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Mycobacterium marinum
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Techniques de diagnostic moléculaire
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Diagnostic
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Génétique
Type d'étude:
Diagnostic_studies
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Etiology_studies
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Prognostic_studies
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Pathology
Année:
2006
Type:
Article