Protective effect of mild hypothermia on astrocytes with traumatic or ischemic injury / 南方医科大学学报
Journal of Southern Medical University
; (12): 61-63, 2010.
Article
Dans Zh
| WPRIM
| ID: wpr-269626
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effect of mild hypothermia on rat astrocytes with traumatic or ischemic injury.</p><p><b>METHODS</b>Rat astrocytes in primary culture were subjected to scratching or hypoxic injury and exposed to normothermia (37 degrees celsius;) or hypothermia (34 or 32 degrees celsius;) for 24 h. The morphology of the astrocytes was evaluated by live/dead staining, and the cell injury was measured by lactate dehydrogenase (LDH) release assay.</p><p><b>RESULTS</b>As the temperature reduced the LDH release rate from the cells in hypoxic group decreased significantly, to (11.48 - or + 1.53)% at 34 degrees celsius; and (3.79 - or + 0.45)% at 32 degrees celsius; as compared to that in normothermia [(33.02 - or + 3.58)%] in the absence of rat white blood cells (WBC) (P<0.001). LDH release rate of the hypoxic cells further decreased in the presence of rat WBC to (51.14 - or + 2.17 )% at 37 degrees celsius;, (19.53 - or + 4.37)% at 34 degrees celsius; and (16.68 - or + 1.47)% at 32 degrees celsius; (P<0.001). In the scratched cells, with or without WBC, LDH release rate showed no significant variation between the 3 temperatures (P>0.05).</p><p><b>CONCLUSION</b>Mild hypothermia offers obvious protective effects on rat astrocytes against ischemic damage but not against mechanical injury.</p>
Texte intégral:
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Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Thérapeutique
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Lésions encéphaliques
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Hypoxie cellulaire
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Cellules cultivées
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Encéphalopathie ischémique
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Astrocytes
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Rat Sprague-Dawley
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Basse température
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L-Lactate dehydrogenase
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2010
Type:
Article