Anti-tumor effect of chemotherapeutic drugs on human gastric cancer cells in vitro and the relationship with Bcl-2 expression / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 276-279, 2008.
Article
de Zh
| WPRIM
| ID: wpr-273848
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate in vitro anti-tumor effect of chemotherapeutic drugs on human gastric cancer cells, and investigate the relationship with Bcl-2 expression.</p><p><b>METHODS</b>Single cell suspension was prepared from fresh gastric cancer tissue and exposed to taxol (Tax), 5-fluorouracil (5-FU), cisplatin (CDDP), adriamycin (ADM), mitomycin (MMC) respectively for 48 hours. Metabolic activity and inhibitory rate of cells were detected by MTT assay. Expression of Bcl-2 was examined with immunohistochemistry.</p><p><b>RESULTS</b>The inhibitory rates of cancer cells exposed to chemotherapeutic drugs were different and Tax, 5-FU, CDDP had remarkably higher rates than ADM and MMC. The lower differentiated gastric cancer cells were more sensitive than the higher ones. Positive expression rate of Bcl-2 was 80% and the positive cells showed resistance to 5-FU, ADM and MMC.</p><p><b>CONCLUSIONS</b>Chemosensitive testing by MTT assay can constitute the prediction for the application of chemotherapeutic drugs individually. Overexpression of Bcl-2 may contribute to multiple drug-resistance of tumors.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
/
Pharmacologie
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Tumeurs de l'estomac
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Tests de criblage d'agents antitumoraux
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Cellules cancéreuses en culture
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Doxorubicine
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Survie cellulaire
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Cisplatine
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Mitomycine
/
Paclitaxel
Limites du sujet:
Adult
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Aged
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Female
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Humans
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Male
langue:
Zh
Texte intégral:
Chinese Journal of Gastrointestinal Surgery
Année:
2008
Type:
Article