Influence of combination of extractum Angelicae Dahuricae Siccum and total alkaloids of Rhizoma Corydalis on pharmacokinetics of tetrahydropalmatine in rats / 药学学报
Yao Xue Xue Bao
; (12): 645-650, 2009.
Article
de Zh
| WPRIM
| ID: wpr-278204
Bibliothèque responsable:
WPRO
ABSTRACT
This paper is aimed to develop a rapid and sensitive HPLC-fluorescence detection (FLD) method for the determination of tetrahydropalmatine (TET) in rats' plasma. The influence of combinations of Extractum Angelicae Dahuricae Siccum (coumarin and volatile oil) and total alkaloids (TA) from Rhizoma Corydalis (TA) on pharmacokinetics of TET in rats was studied. Plasma samples were treated with hexane-isopropanol (95:5) to precipitate the protein, and were determined by HPLC-fluorescence detection. The calibration curve was linear in the range of 2.096-167.68 microg L(-1). The limit of quantification was 2.096 microg L(-1). The method recovery of TET was 94.0%-100.0%. The extract recovery was 72.0%-81.5%. RSDs ofintra- and inter-day precisions were all less than 7.0%. Pharmacokinetics of TET in rats was fitted to two compartments open model after oral administration of TA, TA-volatile oil (VO), TA-coumarin (Cou) and TA-VO-Cou. Compared with TA, AUC(0-t), AUC(0-infinity), MRT(0-t), and MRT(0-infinity) of TET had significant deviation when combined with VO and/or Cou. The determination method is sensitive, specific, accurate, and appropriate for determination of TET in vivo. Coumarin and/or VO combined with TA can prolong the resistance time of TET significantly, delay elimination and enhance bioavailability of tetrahydropalmatine.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
/
Alcaloïdes de type berbérine
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Huile essentielle
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Médicaments issus de plantes chinoises
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Pharmacocinétique
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Chimie
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Rat Sprague-Dawley
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Coumarines
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Angelica
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Rhizome
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Yao Xue Xue Bao
Année:
2009
Type:
Article