Permeability of blood-brain barrier oxygen-glucose deprivation induced by tetramethylpyrazine-puerarin in vitro / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2745-2748, 2010.
Article
Dans Zh
| WPRIM
| ID: wpr-279361
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore permeability of artificial blood-brain barrier (aBBB) by oxygen-glucose deprivation combined (OGD)-induced using tetramethylpyrazine combined with puerarin in vitro.</p><p><b>METHOD</b>Rats were divided into normal control group, model group, tetramethylpyrazine group, puerarin group, tetramethylpyrazine-puerarin group and nimodipine group. Culture rat brain microvascular endothelial cells and astrocytes in vitro and build the OGD-induced aBBB damage model. Evaluate aBBB damage characteristics by TEER, gamma-GT, AKP and LDH. Determine contents of tetramethylpyrazine, puerarin, nimodipine and calculate drug permeating concentration of OGD-induced aBBB model by HPLC.</p><p><b>RESULT</b>Compared with the model, the level of TEER was lower than the control group with significant difference (P < 0.01). The levels of gamma-GT, AKP in tetramethylpyrazine group, tetramethylpyrazine-puerarin group and nimodipine group were higher than the model group, the differences were significant (P < 0.01). Compared with tetramethylpyrazine group or puerarin group, the level of AKP of tetramethylpyrazine-puerarin group increased significantly (P < 0.01). The differences of levels of TEER, gamma-GT, AKP and LDH between tetramethylpyrazine-puerarin group and nimodipinthe group were significant (P < 0.05). Tetramethylpyrazine-puerarin group has a synergistic effect of increasing TEER, gamma-GT, AKP and reducing LDH. The permeating rate in tetramethylpyrazine-puerarin group was higher than tetramethylpyrazine group and puerarin group.</p><p><b>CONCLUSION</b>Tetramethylpyrazine-puerarin can permeate aBBB more easily and protect aBBB. The cause may relate to reducing the permeability of the OGD-induced aBBB.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Oxygène
/
Perméabilité
/
Pharmacologie
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Physiologie
/
Pyrazines
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Barrière hémato-encéphalique
/
Rat Sprague-Dawley
/
Association médicamenteuse
/
Glucose
/
Isoflavones
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2010
Type:
Article