The relationship between alpha-IFN anti-virus treatment and HLA-DRB1*11 gene mononucleotide polymorphism / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 646-648, 2008.
Article
Dans Zh
| WPRIM
| ID: wpr-279715
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between the distribution of mononucleotide polymorphism of five regulation regions of alpha-IFN among HLA-DRB1*11 gene episodes and the therapeutic effects of alpha-IFN treatment in chronic hepatitis B patients.</p><p><b>METHODS</b>One hundred seven chronic hepatitis patients from Nanjing Second Hospital who were treated by alpha-IFN for 12 months and then followed at least six months without the treatment were randomly selected for this regressive analysis. They were grouped into a continuous responsive group and a non-continuous responsive group. Hepatitis B virus X interacting protein gene locus was searched in NCBI. Single nucleotide polymorphism (SNP) gene locus was detected based on a pooling sequencing method. Primer and TaqMan-MGB probes referring to different mononucleotide loci were designed respectively to detect SNP in five regulation regions of alpha-IFN. Then gene sequencing differences between the two groups were analyzed.</p><p><b>RESULTS</b>Among the 107 cases there were 30 cases (28.0%) in the continuous responsive group and 77 cases (71.9%) in the non-continuous responsive group. CT occupation rate in five regulation regions of IFN reached 18.0% in the continuous responsive group and 23.8% in the non-continuous responsive group. AG occupation rate reached 10.8% in the former group and 15.8% in the latter group. The differences in CT and AG between the two groups were significant.</p><p><b>CONCLUSIONS</b>The distribution of mononucleotide polymorphism of five regulation regions of alpha-IFN among HLA-DRB1*11 gene episodes affects the IFN anti-virus treatment. Detecting the gene distribution of mononucleotide in five regulation regions of alpha-IFN helps in predicting the therapeutic effects of alpha-IFN.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Antiviraux
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ADN viral
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Antigènes HLA-DR
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Virus de l'hépatite B
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Analyse de régression
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Résultat thérapeutique
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Interféron alpha
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Hépatite B chronique
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Polymorphisme de nucléotide simple
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Utilisations thérapeutiques
Type d'étude:
Diagnostic_studies
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Prognostic_studies
Limites du sujet:
Adolescent
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Adult
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Humans
langue:
Zh
Texte intégral:
Chinese Journal of Hepatology
Année:
2008
Type:
Article